Mechanism of aerobic transformation of carbon tetrachloride by poplar cells.
Identifieur interne : 004562 ( Main/Exploration ); précédent : 004561; suivant : 004563Mechanism of aerobic transformation of carbon tetrachloride by poplar cells.
Auteurs : Xiaoping Wang [États-Unis] ; Milton P. Gordon ; Stuart E. StrandSource :
- Biodegradation [ 0923-9820 ] ; 2002.
Descripteurs français
- KwdFr :
- Agrobacterium tumefaciens (métabolisme), Antienzymes (pharmacologie), Arbres (métabolisme), Aérobiose (MeSH), Biotransformation (MeSH), Cellules cultivées (MeSH), Cytochrome P-450 CYP2E1 (métabolisme), Cytochrome P-450 enzyme system (métabolisme), Dépollution biologique de l'environnement (MeSH), Inhibiteurs des enzymes du cytochrome P-450 (MeSH), Inhibiteurs du cytochrome P-450 CYP2E1 (MeSH), Microbiologie du sol (MeSH), Oxygène (pharmacologie), Peroxidases (antagonistes et inhibiteurs), Tétrachloro-méthane (métabolisme).
- MESH :
- antagonistes et inhibiteurs : Peroxidases.
- métabolisme : Agrobacterium tumefaciens, Arbres, Cytochrome P-450 CYP2E1, Cytochrome P-450 enzyme system, Tétrachloro-méthane.
- pharmacologie : Antienzymes, Oxygène.
- Aérobiose, Biotransformation, Cellules cultivées, Dépollution biologique de l'environnement, Inhibiteurs des enzymes du cytochrome P-450, Inhibiteurs du cytochrome P-450 CYP2E1, Microbiologie du sol.
English descriptors
- KwdEn :
- Aerobiosis (MeSH), Agrobacterium tumefaciens (metabolism), Biodegradation, Environmental (MeSH), Biotransformation (MeSH), Carbon Tetrachloride (metabolism), Cells, Cultured (MeSH), Cytochrome P-450 CYP2E1 (metabolism), Cytochrome P-450 CYP2E1 Inhibitors (MeSH), Cytochrome P-450 Enzyme Inhibitors (MeSH), Cytochrome P-450 Enzyme System (metabolism), Enzyme Inhibitors (pharmacology), Oxygen (pharmacology), Peroxidases (antagonists & inhibitors), Soil Microbiology (MeSH), Trees (metabolism).
- MESH :
- chemical , antagonists & inhibitors : Peroxidases.
- chemical , metabolism : Carbon Tetrachloride, Cytochrome P-450 CYP2E1, Cytochrome P-450 Enzyme System.
- metabolism : Agrobacterium tumefaciens, Trees.
- chemical , pharmacology : Enzyme Inhibitors, Oxygen.
- Aerobiosis, Biodegradation, Environmental, Biotransformation, Cells, Cultured, Cytochrome P-450 CYP2E1 Inhibitors, Cytochrome P-450 Enzyme Inhibitors, Soil Microbiology.
Abstract
The biochemical mechanism of carbon tetrachloride transformation by poplar cells was investigated using an axenic poplar cell culture. After one-day incubations of poplar cells under aerobic conditions, about 1.5% of dosed carbon tetrachloride was transformed to carbon dioxide, about 0.001% to chloroform and about 3% of the carbon was bound to insoluble poplar cellular materials. The production of carbon dioxide increased under aerobic conditions while the formation of chloroform and cell binding of carbon tetrachloride-carbon was enhanced under anaerobic conditions. Both carbon dioxide production and cell binding were significantly inhibited by a general inhibitor of cytochrome P-450 activity (carbon monoxide) and by specific P-450 2E1 inhibitors (chlorzoxazone, isoniazid, 4-methylpyrazole and 1-phenylimidazole). However, no inhibitory effects were observed when the cells were incubated in the presence of lignin peroxidase inhibitors (NaVO3 and 3-amino-1,2,4-triazole). These results suggest that an enzyme similar to mammalian cytochrome P450-2E1 is involved in the metabolism of carbon tetrachloride by poplar cells. This study demonstrates an environmental biodegradative process for carbon tetrachloride that operates under aerobic conditions.
DOI: 10.1023/a:1022397121365
PubMed: 12688582
Affiliations:
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Gordon</name></author><author><name sortKey="Strand, Stuart E" sort="Strand, Stuart E" uniqKey="Strand S" first="Stuart E" last="Strand">Stuart E. Strand</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2002">2002</date><idno type="RBID">pubmed:12688582</idno><idno type="pmid">12688582</idno><idno type="doi">10.1023/a:1022397121365</idno><idno type="wicri:Area/Main/Corpus">004488</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">004488</idno><idno type="wicri:Area/Main/Curation">004488</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">004488</idno><idno type="wicri:Area/Main/Exploration">004488</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Mechanism of aerobic transformation of carbon tetrachloride by poplar cells.</title><author><name sortKey="Wang, Xiaoping" sort="Wang, Xiaoping" uniqKey="Wang X" first="Xiaoping" last="Wang">Xiaoping Wang</name><affiliation wicri:level="4"><nlm:affiliation>College of Forest Resources, University of Washington, Seattle, WA 98195, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>College of Forest Resources, University of Washington, Seattle, WA 98195</wicri:regionArea><placeName><region type="state">Washington (État)</region><settlement type="city">Seattle</settlement></placeName><orgName type="university">Université de Washington</orgName></affiliation></author><author><name sortKey="Gordon, Milton P" sort="Gordon, Milton P" uniqKey="Gordon M" first="Milton P" last="Gordon">Milton P. Gordon</name></author><author><name sortKey="Strand, Stuart E" sort="Strand, Stuart E" uniqKey="Strand S" first="Stuart E" last="Strand">Stuart E. Strand</name></author></analytic><series><title level="j">Biodegradation</title><idno type="ISSN">0923-9820</idno><imprint><date when="2002" type="published">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aerobiosis (MeSH)</term><term>Agrobacterium tumefaciens (metabolism)</term><term>Biodegradation, Environmental (MeSH)</term><term>Biotransformation (MeSH)</term><term>Carbon Tetrachloride (metabolism)</term><term>Cells, Cultured (MeSH)</term><term>Cytochrome P-450 CYP2E1 (metabolism)</term><term>Cytochrome P-450 CYP2E1 Inhibitors (MeSH)</term><term>Cytochrome P-450 Enzyme Inhibitors (MeSH)</term><term>Cytochrome P-450 Enzyme System (metabolism)</term><term>Enzyme Inhibitors (pharmacology)</term><term>Oxygen (pharmacology)</term><term>Peroxidases (antagonists & inhibitors)</term><term>Soil Microbiology (MeSH)</term><term>Trees (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Agrobacterium tumefaciens (métabolisme)</term><term>Antienzymes (pharmacologie)</term><term>Arbres (métabolisme)</term><term>Aérobiose (MeSH)</term><term>Biotransformation (MeSH)</term><term>Cellules cultivées (MeSH)</term><term>Cytochrome P-450 CYP2E1 (métabolisme)</term><term>Cytochrome P-450 enzyme system (métabolisme)</term><term>Dépollution biologique de l'environnement (MeSH)</term><term>Inhibiteurs des enzymes du cytochrome P-450 (MeSH)</term><term>Inhibiteurs du cytochrome P-450 CYP2E1 (MeSH)</term><term>Microbiologie du sol (MeSH)</term><term>Oxygène (pharmacologie)</term><term>Peroxidases (antagonistes et inhibiteurs)</term><term>Tétrachloro-méthane (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Peroxidases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Carbon Tetrachloride</term><term>Cytochrome P-450 CYP2E1</term><term>Cytochrome P-450 Enzyme System</term></keywords><keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Peroxidases</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Agrobacterium tumefaciens</term><term>Arbres</term><term>Cytochrome P-450 CYP2E1</term><term>Cytochrome P-450 enzyme system</term><term>Tétrachloro-méthane</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antienzymes</term><term>Oxygène</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Enzyme Inhibitors</term><term>Oxygen</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Aerobiosis</term><term>Biodegradation, Environmental</term><term>Biotransformation</term><term>Cells, Cultured</term><term>Cytochrome P-450 CYP2E1 Inhibitors</term><term>Cytochrome P-450 Enzyme Inhibitors</term><term>Soil Microbiology</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Aérobiose</term><term>Biotransformation</term><term>Cellules cultivées</term><term>Dépollution biologique de l'environnement</term><term>Inhibiteurs des enzymes du cytochrome P-450</term><term>Inhibiteurs du cytochrome P-450 CYP2E1</term><term>Microbiologie du sol</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The biochemical mechanism of carbon tetrachloride transformation by poplar cells was investigated using an axenic poplar cell culture. After one-day incubations of poplar cells under aerobic conditions, about 1.5% of dosed carbon tetrachloride was transformed to carbon dioxide, about 0.001% to chloroform and about 3% of the carbon was bound to insoluble poplar cellular materials. The production of carbon dioxide increased under aerobic conditions while the formation of chloroform and cell binding of carbon tetrachloride-carbon was enhanced under anaerobic conditions. Both carbon dioxide production and cell binding were significantly inhibited by a general inhibitor of cytochrome P-450 activity (carbon monoxide) and by specific P-450 2E1 inhibitors (chlorzoxazone, isoniazid, 4-methylpyrazole and 1-phenylimidazole). However, no inhibitory effects were observed when the cells were incubated in the presence of lignin peroxidase inhibitors (NaVO3 and 3-amino-1,2,4-triazole). These results suggest that an enzyme similar to mammalian cytochrome P450-2E1 is involved in the metabolism of carbon tetrachloride by poplar cells. This study demonstrates an environmental biodegradative process for carbon tetrachloride that operates under aerobic conditions.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12688582</PMID><DateCompleted><Year>2003</Year><Month>10</Month><Day>21</Day></DateCompleted><DateRevised><Year>2019</Year><Month>11</Month><Day>07</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0923-9820</ISSN><JournalIssue CitedMedium="Print"><Volume>13</Volume><Issue>5</Issue><PubDate><Year>2002</Year></PubDate></JournalIssue><Title>Biodegradation</Title><ISOAbbreviation>Biodegradation</ISOAbbreviation></Journal><ArticleTitle>Mechanism of aerobic transformation of carbon tetrachloride by poplar cells.</ArticleTitle><Pagination><MedlinePgn>297-305</MedlinePgn></Pagination><Abstract><AbstractText>The biochemical mechanism of carbon tetrachloride transformation by poplar cells was investigated using an axenic poplar cell culture. After one-day incubations of poplar cells under aerobic conditions, about 1.5% of dosed carbon tetrachloride was transformed to carbon dioxide, about 0.001% to chloroform and about 3% of the carbon was bound to insoluble poplar cellular materials. The production of carbon dioxide increased under aerobic conditions while the formation of chloroform and cell binding of carbon tetrachloride-carbon was enhanced under anaerobic conditions. Both carbon dioxide production and cell binding were significantly inhibited by a general inhibitor of cytochrome P-450 activity (carbon monoxide) and by specific P-450 2E1 inhibitors (chlorzoxazone, isoniazid, 4-methylpyrazole and 1-phenylimidazole). However, no inhibitory effects were observed when the cells were incubated in the presence of lignin peroxidase inhibitors (NaVO3 and 3-amino-1,2,4-triazole). These results suggest that an enzyme similar to mammalian cytochrome P450-2E1 is involved in the metabolism of carbon tetrachloride by poplar cells. 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UI="D010544">Peroxidases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber><NameOfSubstance UI="C042858">lignin peroxidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.14.13.-</RegistryNumber><NameOfSubstance UI="D019392">Cytochrome P-450 CYP2E1</NameOfSubstance></Chemical><Chemical><RegistryNumber>S88TT14065</RegistryNumber><NameOfSubstance UI="D010100">Oxygen</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000332" MajorTopicYN="N">Aerobiosis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001673" MajorTopicYN="N">Biodegradation, Environmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001711" 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MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004791" MajorTopicYN="N">Enzyme Inhibitors</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010100" MajorTopicYN="N">Oxygen</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010544" MajorTopicYN="N">Peroxidases</DescriptorName><QualifierName UI="Q000037" MajorTopicYN="N">antagonists & inhibitors</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012988" MajorTopicYN="N">Soil Microbiology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2003</Year><Month>4</Month><Day>12</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2003</Year><Month>10</Month><Day>22</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2003</Year><Month>4</Month><Day>12</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">12688582</ArticleId><ArticleId IdType="doi">10.1023/a:1022397121365</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Washington (État)</li></region><settlement><li>Seattle</li></settlement><orgName><li>Université de Washington</li></orgName></list><tree><noCountry><name sortKey="Gordon, Milton P" sort="Gordon, Milton P" uniqKey="Gordon M" first="Milton P" last="Gordon">Milton P. Gordon</name><name sortKey="Strand, Stuart E" sort="Strand, Stuart E" uniqKey="Strand S" first="Stuart E" last="Strand">Stuart E. Strand</name></noCountry><country name="États-Unis"><region name="Washington (État)"><name sortKey="Wang, Xiaoping" sort="Wang, Xiaoping" uniqKey="Wang X" first="Xiaoping" last="Wang">Xiaoping Wang</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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